Product Name :
Phospho-AKT (S473) Recombinant Rabbit Monoclonal Antibody [SY28-05]
Predicted band size :
56 kDa
Observed band size :
56 kDa
Synonyms:
AKT 1 antibody AKT antibody AKT1 antibody AKT1_HUMAN antibody MGC99656 antibody PKB antibody PKB-ALPHA antibody PRKBA antibody Protein Kinase B Alpha antibody Protein kinase B antibody 展开
Function :
RAC(Rho family)-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases that contain SH2 (Src homology 2-like) protein domains. It is commonly referred to as PKB, or by both names as “Akt/PKB”. The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase.2-Cyclopropylethanol Chemscene In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser473 of human AKT1.
Species reactivity:
Human, Mouse, Rat, Dog
Verify application:
WB, IF-Cell, IF-Tissue, IHC-P, IHC-Fr
Coupling :
unconjugated
Clone number:
SY28-05
RRID:
AB_2940863
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.173315-56-5 supplier Aliquot store at -20℃ or -80℃.PMID:33515558 Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 56 kDa
Positive control :
MCF7 treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 100ng/mL PDGF for 1 hour cell lysate, C6 treated with 100nM Calyculin A for 30 minutes cell lysate, HEK-293 cell lysate, HeLa cells treated with 100nM Calyculin A for 30 minutes, NIH/3T3 cells treated with 100ng/mL PDGF for 1 hour, C6 cells treated with 100nM Calyculin A for 30 minutes, human breast cancer tissue, mouse lung tissue, rat brain tissue, mouse hippocampus tissue, mouse cerebral cortex tissue, mouse brain tissue.