Product Name :
Vimentin Recombinant Mouse Monoclonal Antibody [A6-C1-R]
Predicted band size :
54 kDa
Observed band size :
54 kDa
Synonyms:
CTRCT30 antibody Epididymis luminal protein 113 antibody FLJ36605 antibody HEL113 antibody VIM antibody VIME_HUMAN antibody Vimentin antibody
Function :
Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. In essence, vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin has been shown to eliminate toxic proteins in JUNQ and IPOD inclusion bodies in asymmetric division of asymmetric division of mammalian cell lines. It has been used as a sarcoma tumor marker to identify mesenchyme. Methylation of the vimentin gene has been established as a biomarker of colon cancer and this is being utilized in the development of fecal tests for colon cancer. High levels of DNA methylation in the promotor region have also been associated with markedly decreased survival in hormone positive breast cancers.
Antibody Type:
Recombinant Mouse Monoclonal Antibody
Immunogen :
Synthetic peptide within Human Vimentin aa 1-50 / 466.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell, IHC-P
Coupling :
unconjugated
Clone number:
A6-C1-R
RRID:
AB_3096827
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.Price of Quinoline-6-sulfonyl chloride Aliquot store at -20℃.1-Cyclopentyl-1h-1,2,4-triazole site Avoid repeated freeze / thaw cycles.PMID:33626958
Storage buffer solution:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG1
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 54 kDa
Positive control :
HeLa cell lysate, C2C12 cell lysate, L6 cell lysate, HeLa, human appendix tissue, human kidney tissue, human liver tissue.